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KMID : 0379120080360020189
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Korean Journal of Mycology
2008 Volume.36 No. 2 p.189 ~ p.195
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Isolation and Functional Analysis of the silA Gene That Controls Sexual Development in Response to Light in Aspergillus nidulans
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Han Sang-Yong
Ko Jin-A
Kim Jong-Hak
Han Kyu-Yong
Han Kap-Hoon
Han Dong-Min
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Abstract
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When a homothallic ascomycete Aspergillus nidulans is exposed to visible light, cleistothecial development is inhibited. The light response of development in A. nidulans implies the existence of delicate regulation process including reception and translocation of light signaling and determination of development. Previously, mutants that could develop cleistothecia even in the presence of relatively intensive visible light were isolated and several complementation groups were identified. A gene that was able to complement the silA98 mutation, which was responsible for preferred cleistothecia development under visible light, was isolated from AMA-NotI genomic library. The silA gene retained in the 4.3 kb recovered genomic library DNA has an open reading frame (ORF) consisted of 2,388 bp nucleotides, interrupted by 3 introns and consequently encoding 795 amino acids. The putative SilA carries a binuclear cluster motif at N terminus and shows high amino acid sequence similarity to Aro80p of Saccharomyces cerevisiae. Deletion mutants of silA showed a strong induction of sexual development under visible light, indicating that SilA is involved in the negative regulation of sexual development in response to the light.
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KEYWORD
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Aspergillus nidulans, Light, Sexual development, silA, Transcription factor
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